Sunday , 3 March 2024
Home Isoform-resolved mRNA profiling of ribosome load defines interplay of HIF and mTOR dysregulation in kidney cancer – Nature Structural & Molecular Biology

Isoform-resolved mRNA profiling of ribosome load defines interplay of HIF and mTOR dysregulation in kidney cancer – Nature Structural & Molecular Biology

HP5 encompasses two key features. First, through the use of external RNA standards, it robustly measures ribosome load of mRNAs. Second, by the exclusion of mRNA or cDNA purification steps before the first PCR amplification and multiplexing of samples at an early stage of the protocol, the method enables the processing of a large number of samples. (Fig. 1a and Extended Data Fig. 1).

 

Continue reading your article with a FrontPage News Subscription

Already a Subscriber? Sign In  

OR
VIEW SUBSCRIPTION OPTIONS
Support independent journalism by subscribing