Sunday , 3 March 2024
Home Science Cohesin is required for long-range enhancer action at the Shh locus – Nature Structural & Molecular Biology
Science

Cohesin is required for long-range enhancer action at the Shh locus – Nature Structural & Molecular Biology

Here, we exploit synthetic transcriptional activators, coupled with the acute degradation of CTCF or cohesin, to investigate mechanisms of enhancer action at various distances, using the sonic hedgehog signaling molecule (Shh) locus as a paradigm. In mouse embryonic stem cells (mESCs), we show that cohesin, but not CTCF, is required for activation of Shh by enhancers located many hundreds of kilobases upstream, but is dispensable for activation from an enhancer located closer to the target gene.

 

Continue reading your article with a FrontPage News Subscription

Already a Subscriber? Sign In  

OR
VIEW SUBSCRIPTION OPTIONS
Support independent journalism by subscribing